Pasteur's swan-neck flask ends spontaneous generation
In the early 1860s Louis Pasteur boiled broth in flasks whose long necks he had bent into an S-curve. The necks stayed open to the air, but dust and microbes settled in the bend. The experiment showed that microorganisms come from other microorganisms carried on dust, not from the air or the broth itself, which laid the groundwork for germ theory.
